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Curriculum - Prof.ssa Maria Luigia PALLOTTA

Curriculum

Maria Luigia Pallotta, Biochemist

Full Time Permanent Researcher  (SSDBIO/10) /Aggregate Professor,

Department of Medicine and Health Sciences, "V. Tiberio"-

University of Molise- Via deSanctis-86100 Campobasso (ITALY).

Office Tel:+39.874.404673;Fax+39.874.404778.

Mobile phone +39. 392. 1528730 -

E-mail: pallotta@unimol.it 

pallottamarialuigia@gmail.com

-http://docenti.unimol.it/index.php?u=pallotta

  • She received her Agricultural Sciences  Master Degree from University of Bari-Italy (June1986) score 110/110, with dissertation on Food Chemistry- experimental thesis  -more 2 years at  lab of prof Pallavicini, Head of Dept Prof Catalano "Preparazione ed analisi di coprecipitati ottenuti da miscele di proteine di soia e siero di latte trattate con proteasi "-.
  • (i.e. Protein Isolation from food matrix,  protein assay, N determination in micro-Kjeldahl digests,  Protein purification,  Enzymatic Assay, Kinetics, Inhibition, SDS-PAGE, Disc Electrophoresis, Isoelectrofocusing, HPLC, et al.)  
  •  
     She taught some years Chemistry, Biology and Astronomical Geography at high school, (i.e. Classical Liceo in Ostuni and Fasano, Brindisi, Italy) and,
  • concurrently, she attended Pallavicini ‘s lab, at University of Bari & improved her knoweledge on  Food Chemistry & Technology.
  • Moreover she worked, for free,  like pubblicist & designer together her husband TI -Urban Planner & Landscape Architect +SpatialAnalyst GIS -GIS Web-
  • & for organizing charity events with her friends (on 7 March, 1991 arrived to Brindisi 25000 people from Albania, it was a major  emergency)
  •  
  • After winning a public competition, she joined at University of Molise, October 1991
  • Dipartimento Agricoltura, Ambiente e Alimenti from 1991- to 1993
  • Dipartimento  Scienze Animali, Vegetali, Ambientali, from 1994-to 2001
  • Centro Ricerca e Servizio di Ateneo per la Formazione “G.A. Colozza”  from 2002- to 20005
  • Dipartimento di Medicina e di Scienze della Salute from 2005 to 2019
  •  
  • National scientific eligibility  as -Full-time position- as Permanent Researcher (Settore Concorsuale G05, Microbiology,Technology & Agricultural Chemistry, bando PDN 205, del 29/10/1993 GUN 98 bis del 10/12/93) University of Udine,  oral score 10/10
  • Permanent Researcher (Settore Concorsuale BIO/10 Biochemistry) 2002

  •  

  • Training

  • Proteins Separation– Institute of Neurobiology,  National Research Council (CNR)–Rome 1993 

  • Capillary Electrophoresis –Institute of Neurobiology National Research Council (CNR)–Rome 1993 

  • Molecular Biology in diagnostic - BioResearch Center -Milan 1993

  • The Digoxigenin System for the Labeling and Detection of Nucleic Acids, National Research Council (CNR)  Bari 1994

  • Molecular Biology–Advanced course, Biomedical International Science Park  San Raffaele Milan, 1995 

  • LC/MS advanced techniques in HPLC Perkin Elmer, Rome 1996 

  • 7th European PCR Seminar Tour, Università Cattolica Sacro Cuore, Faculty of Medicine, Rome 1996 

  • HLA typing by sequencing: comparing experiences, Center of Advanced Biotechnology, San Martino’s   Hospital, Genoa 1997  

  • Bio-technology applied to molecular diagnostic Center of studies on Mitochondria and Energetic Metabolism  - SIBIOC Trani (Bari) 1997

  • Quantitative PCR- State of Art and new technology-Group of Clinical Biochemistry, University of Florence, 1998

  • Progetto Proteoma -Techniche integrate per lo studio di proteine, Perkin Elmer, Roma 1998

  • Italian–Japanese Bilateral Meeting on Bioenergetics, University of Bari, 1998. 

  • Shaping the future: the impact of structural biology- M. Brunori (La Sapienza), R. Huber (Nobel Laureate), J. Walker (Nobel Laureate), D. Wiley (Harvard University), K.Holmes (Max Planck Institut fur Medizinische Forschung), S. Harrison. Rome 1999 

  • Genes and Responsibility ‘s civil Scientist - G. Martini,G. Corbellini, E. Boncinelli, J. Beckwith, U. di Porzio. Italian Institute for Philosophical Studies, Naple, 2000

  • The next revolution: bio e biotec- J. Meldolesi, B. Sitia, F. Terragni, House of Culture, Milan 2000.

  • Structure and dynamics of RNA and DNA viruses –J.Johnson, the SCRIPP Research Institute La Jolla, L Gehrke Harvard Medical School,  Boston December 2000.

  • MFCS/win Basic operator training- Braun Biotech International Biostat B Bioreactor System that covers a wide range of applications, extending from use as a flexible basic unit for preclinical research to a fully qualified system that complies with the requirements of a validated GMP environment.  Campobasso, 2001

  • Microarray User’s Meeting-Istituto Nazionale Malattie infettive “L. Spallanzani”, Roma, 2001.

  • 1st International Meeting on Yeast Apoptosis, University of Minho, Braga, Portugal  2002. 

  • Yeast as a test tube for pharmacological drugs screening University of Washington, Seattle, WA. July - August, 2004

  • Mobility: a space for the exploitation of research. Rome, National Research Council (CNR) , 2005 

  • World Day of Intellectual Property, Ministry of Economic Development, 2005, Rome.

  • Microfluids: lab-on-a-chip technology for protein and nucleic acid assays  “ Experion System” – Campobasso, 2006 

  • Nuove tecnologie e Sviluppi nell’analisi del Proteoma, Medical School,Università degli Studi di Roma "Tor Vergata"2006 

  •  Database searching-Basic Rules & Tools-FEBS working group on Teaching Biochemistry in Europe, Istanbul 2006
  • 3rd International Conference on Functional Genomics of Aging, ISMETT Palerm 2007 (only listener)
  • 3rd Cell Stress Society International Congress on Stress responses in Biology and Medicine and 2nd Word Conference of Stress Budapest 2007 
  • Use of ultra-performance liquid chromatography (UPLC) for making real-time pooling decisions for process chromatography-Waters, CB 2008
  • EndNote and EndNote Web, Scientific Library, National Cancer Institute,  Frederick, Maryland, US, April 2014  
  • Research Integrity Accademia Nazionale dei Lincei Rome, March 2015
  • Transdisciplinary Research in Energetics and Cancer (TREC) Workshop, ISBNPA Cancer Prevention & Management SIG, Edinburgh June 2015
  • Strategie di utilizzo e le novità della piattaforma bibliografico/citazionale per la Ricerca 'Web of Science' + InCites Benchmarking & Analytics,  2019, Biblioteca di Ateneo,  Campobasso
  • WEBINAR 2020
  • Immunometabolic Analysis of Pseudomonas aeruginosa Pulmonary Infection
  • Sebastián A Riquelme, PhD
    Columbia University Medical Center
  • Mitochondrial Function using MitoPlates™ Brendan Lewis
    Specialist Biolog, Inc.
  • Analysis of Microbial Genomic and Phenotypic Data using DuctApe Software
  • Marco Galardini, Ph.D. Biolog
  • Assessing Functional Traits of Microbial Communities using EcoPlates™".
  • Understanding Antibiotic Mechanism of Action with Phenotype Microarrays™ and Machine Learning, JasonH.Yang,Ph.D.
    The Use of Biolog GEN III MicroPlates™ in the Undergraduate Unknown Microbe Identification Project, Jeffrey Newman, PhD Biology Professor Lycoming College
  • Biomeccanica e valutazione del profilo di attivazione muscolare 
  • Profilo forza-velocità e caratterizzazione funzionale
    Applicazioni pratiche e variazioni nel piano di allenamento annuale, 2020 ATS - Giacomo Catalani Editore, September, 16
  • English language courses
  • English language course- during all primary schooling , with Profssa Savastano, Bojano CB
  • English language course-London July-August 1978
  • English language course-Centre of English Studies- July-August 1995 Dublin Ireland English language course for Intermediate learners”
  • Operational Program 1994/99”Research, Technological development and Advanced Training”  From May To October 2001, 100/100 score ultimate test,  Campobasso  Italy
  • English language course for High Intermediate learners, Malta January 2002 
  • Advanced course- English Language Center-Boston Massachusetts May-June 2005. 
  •  
  • Research topics:
  • Bioenergetics, metabolism and transport of metabolic biomolecules, nucleotides, cofactors and coenzymes by isolated and bioenergically active  mitochondria under physiological and stressful conditions;
  • Innovative  biomarkers for improving  diagnostics in 4Pmedicine 
  • Microbiota & nutaceuticals
  •  
  • Realization and organization of the following symposium
  • Meeting with joined sessions GIBB (Italian Group of Bioenergetics and Biomembranes)-Membrane and Bioenergetic Group of SIB (Italian Sociaty of Biochemistry), Prof Passarella/group team, Italy, June 1997.
  • From research to calendar Multiple Sclerosis  Supervisor Prof Pallotta 2015-2020 May 2015 Campobasso
  •  
  • Membership in scientific societies:
  • SIB (Italian Sociaty of Biochemistry)and GIBB (Italian Group of Bioenergetics and Biomembranes) -since 1999-;
  • Women in Science (since  2000)
  • EUSTIM (European Society for Translational Medicine)2016,
  • ISM (International Society of Microbiota)  2016
  • ISHS 2019 (International Society Horticultural Sciences)
  • ESCI 2019 (European Society of Clinical Investigation)
  • American Association for Avancement of the Sciences Membership from 2020/2022
  • Royal Society of Medicine September 2020
  • SiBioc 2019Società Italiana di Biochimica Clinica e Biologia Molecolare chimica
  • Member of the council of PhD in Applied Biochemistry and Chemistry, University of Molise (from 2003 to 2005);
  • Member of the council of PhD in Health Science, University of Molise (from 2005 to 2014)
  • co-mentoring  thesis PhD abroad
  • Teaching activity   from 2002   to 2019
  • Fundamentals of Instrumental Biochemistry (from 2002 to 2003)
  • Propedeutics biochemistry (from 2002 to 2014) (SMS)
  • Biochemistry, (untill now) (SMS)
  • Bioinformatics, (STA)
  • Amino Acid metabolism, (STA)
  • Nucleotide metabolism, (STA)
  • Applied Biochemistry  (corso di laurea specialistica in Biotecnologie, interfacoltà Scienze Tecnologie Alimentari-STA  & Scienze Biologiche) 
  • Biochemistry (STA)
  • +4340 Student from  AA 2002/2019+ other academic experiences from 1991to 2002 Students???
  • Academic appointments
  • She was Member of Senate (INTEGRATED) of University of Molise
  • (From DECEMBER 1999  to January 2003)
  • She was Member of Senate of University of Molise 2003-2006, Area Activities A
  • Member of Committee for recognition credit transfer at the Faculty of Health Sciences, University of Molise, from 2003  to 2010
  • Member of Committee for the evaluation foreign students  at the Faculty of Health Sciences, University of Molise, from 2004 to 2009.
  • She was invited academic guest at Metabolism and Cancer susceptibility, Center for Cancer Research, NIH, Frederick, Washington MD United States from April to May 2014 (Research: NAD(P)+/NAD(P)H pool studies by means of Fluorescence-Based Techniques in cancer cells).
  • E’ stata nominata componente delle commissioni per il conferimento dei titoli di dottore di ricerca in Biochimica e Biologia molecolare (XVI° ciclo) e Tecnologie cellulari e Molecolari in Fisiologia (XVI° ciclo), Università degli Studi di Bari (aa 2003-04);
  • E’ stata nominata componente delle commissioni per il conferimento dei titoli di dottore di ricerca in Tecnologie cellulari e Molecolari in Fisiologia (XVIII° ciclo), Università degli Studi di Bari (aa 2005-06).
  • E’ stata componente della commissione giudicatrice per il conferimento di N.1 assegno di ricerca in Biochimica Progetto TIORCAS (Trasferimento Innovazione ed Organizzazione nella Ricerca, nella Cultura, nell’Ambiente e nella Sanità), concepito nell’ambito della iniziativa comunitaria INTERREG III/A Transfrontaliero Adriatico (Università degli Studi del Molise)
  • E’ stata componente della commissione giudicatrice per il conferimento di N.1 Ricercatore a tempo determinato per il s.s.d. M-EDF/01 “Metodi e didattiche delle attività motorie” presso la Facoltà di Scienze del Benessere dell’Università degli Studi del Molise, with Prof Ferrara (Medicina Interna) &  Prof Daniele (Biochimica), 2005 
  • E’ stata componente della commissione giudicatrice per il conferimento di N.1 assegno di ricerca in Biochimica “ Il canale mitocondriale del potassio in specie vegetali: effetti sulla sintesi di ATP e ruolo dei meccanismi di difesa dagli stress biotici”, with Prof Capitanio (ordinario Biochimica) + prof Pastore (associato Biochimica), Università degli Studi di Foggia, 13.04.2006.
  • E’ stata componente ( with Prof Ferrara &  dottssa Cacchione )  della commissione preposta alla valutazione delle offerte pervenute di cui all’oggetto: trattativa privata per fornitura, consegna e personalizzazione di materiale sportivo per il corso di Laurea in Scienze Motorie; Università degli Studi del Molise (Giugno 2004). 
  • E’ stata proponente di un progetto di didattica integrativo dal titolo”Attività integrativa, propedeutica e di recupero volta a migliorare qualitativamente l’offerta didattica e finalizzata ad assicurare ad un maggior numero di studenti la rapida progressione nelle attività curriculari previste nel corso di laurea frequentato e ridurre abbandoni e ritardi”, per
    un numero di ore pari a 44 presso il Centro Ricerca e Servizi di Ateneo per la Formazione G.A. Colozza, approvato dal Senato Accademico dell’Università degli Studi del Molise e realizzato nell’anno accademico 2002/2003.
  • E’ stata nominata Presidente della Commissione preposta alla preparazione del test“Selezione per l’accesso al primo anno del Corso di Laurea in Scienze Motorie e Sportive- per l’anno accademico 2010/2011
  • e componente in tutti gli anni accademici successivi incluso AA 2018/2019
  •  
  •  
  • Research Funding
  • COST Action CA15203  EGnaiger MitoEAGLEEvolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mappingPallotta ML MitoPedia topics: EAGLE COST: Member COST WG1: WG12018/19
  • Unimol, Dept Medicine  2016 Candidate probiotic strains, with health-improving properties, isolated from traditional Italian dairy products PI MLPallotta, with Prof Colavita & prof Gaspari
  • PRIN 2006 Genomic and proteomic approach to investigate lateral root formation in woody plant under environmental stress-component Unity of Molise University
  • INTERREG programma TIORCAS – “Trasferimento Innovazione ed Organizzazione nella Ricerca, nella Cultura, nell’Ambiente e nella Sanità”
  • (NB She after having elaborated and written the project left for irremediable incompatibility in the management of the funds, 2005?)
  • PRIN 2004 Cross talk between organelles in response to oxidative stress and programmed cell death in plant - component Unity of Molise University
  • FIRB 2003 Metodi teorici e computazionali per lo studio dell’interazione proteina-proteina e proteina-DNA (by means of  BLOSpectrum software)- component Unity of Molise University-
  • PRIN 2001 Bioenergetics: genetic, biochemistry and physiopathology
  • CNR 2000 Subcellular organelles and regulation of metabolism PRIN 1999 Bioenergetics and membrane transport -component Unity of Molise University-.
  • Mi.C.I.A. Project “Cereal Improvement for Agro-Industrial Innovations” Ministry of Agricultural Policy Project POP “Evaluation of antioxidant activity of Agro-Industrial products” Region of Molise -component Unity of Molise University.
  • +++et al.(by private mail)
  • Reviewer Activity 

  • Several Journal (high & low I.F.)

  • Journal of Biological Methods (JBM) Board Member, San Francisco, California, US from 2014
  • Different Journals  on Microbiology/Medicine/ Biotechnology Issues within   Editorial Team & technical expertise for evaluition in Academic Journals next  IF on Scopus/IsiWEb
  •  
  • More of 33 years of experiences on:
  •  
  • HPLC fluorescent   and UV detection

  • Spectrofluorimetric techniques & technologies

  • PCR, Cloning techniques analysis of gene and protein expression, i.e. enzymatic activity assays, cell viability and cytotoxic assays, 

  • Generation of knock out and over-expressing yeast strains

  • Electrophoresis

  • Immunochemistry

  • Polarographic oxygen analyzer

  • Statistical analysis; 
  • ExCellent  command of Microsoft office tools (Word, Excel, Power Point);
  • Excellent knowledge of graphic design applications ( Corel Draw, Paint, Photoshop, BioDraw et al.)
  • Excellent knowledge of applications on Enzymatic kinetics & Inhibition  (Grafit, ecc)
  • EXPERTISE:

  • Mitochondria

  • Flavin homeostasis

  • NAD+ synthesis via NMN 1-3  enzymes

  • L-Proline metabolism,

  • D-Lactate metabolism and  transport

  • Nucleotide pool (i.e. ATP, ADP, AMP, AMPc, GDP, GTP, NMN)

  • NAD(P) + / NAD(P)H  cytosolic and mitochondrial pool

  • Riboflavin + FMN+FAD synthesis, catabolism and transport in mitochondria

  • GSH/GSSG assay

  • ROS and innovative  markers in stress conditions

  • Transmembrane electrical potential (ΔΨ) assays in different metabolic conditions

  • Specific biomarkers for improving  4P diagnostics in medicine

  • Fecal Microbiota Transplantation   

  •  

  • Knowledge, skills & attitudes  

  • oral  & written communication skills;

  • mentoring activities;

  • team working skills; 

  • problem solving;

  • work planning and organization;
  • grant writing and project management;
  • courses, meeting and events organization;
  • teaching activities;
  • scientific dissemination activities (writer /copywriter on different blog, for references pallotta@unimol.it, or pallottamarialuigia@gmail.com)
  • press office 
  • only with people good will'

     

  • for references on researches' activity 

  • Prof Cosmo Pallavicini (lab's training & mentoring) Università di Bari

  • Profssa Claudia Sorlini Unimol.it; Università di Milano

    Prof Franco Dellaglio Unimol.it; Università Di Piacenza, Cattolica

    Profssa Sandra Torriani Università di Verona

    Prof Valerio Giaccone Unimol; Università di Torino

    Prof  Antonio Girolami Unimol.it; Universià di Napoli

    Prof Salvatore Passarella Unimol.it; Università di Bari

    Prof Donato Chiatante Unimol.it; Università Varese Insubria

    Prof Aurelio de Sanctis Università delle Marche

    Prof ssa Maria Barile Universitò di Bari

    Prof Donato Pastore Unimol; Università di Foggia

    dottssa Daniela Valenti  Unimol.it CNR, Bari

    +et al

    NIH Scientist Emeritus James M. Phang, M.D.,Center for Cancer Research
    National Cancer InstituteFrederick, Washington, MD 21702-1201

    Prof Erich Gneiger Gentle Scientist MD,PhD, Medical University of Innsbruck, D. Swarovski Research Laboratory, CEO ORABORO INSTs

    She loved/s studying, reading, exhibitions, travelling, nature.

    She has a great ecological coscienza and partecipa attivamente a marce e convegni sulla conservazione del pianeta, agricoltura biodinamica e circolare, e energie alternative/rinnovabili, biotecnologie trandisciplinari e innovative.

    Ama la fisica nucleare e l’ottica e durante i primi anni nel laboratorio a CB, ha contribuito a realizzare uno spettrofotometro a doppia lunghezza d’onda, doppio raggio, assemblando assieme, a tandem, due spettrofotometri, al fine di analizzare i citocromi della catena respiratoria mitocondriale nelle due forme, ridotte e ossidate, contemporaneamente. La strumentazione è stata ampiamente utilizzata durante il corso interdipartimentale di Biochimica Applicata, tenuto dalla stessa in numerosi AA, nelle esercitazioni tenute dalla stessa per il corso di Biochimica del Prof Passarella ed ha prodotto contributi a convegni scientifici internazionali e altri articoli scientifici (see BBA et Bioenergetics, Pallotta et al, 2004, e altri pubblicati dai membri del team di ricerca del Prof Passarella a Cb e Ba)

    Iscritta alla CGIL dal 1991, poi lavoratori della conoscenza.

    E’ molto sensibile ai temi sociali sin da piccola ha partecipato a convegni, marce, raduni alfine di raggiungere una equità universale e un accettabile livello di libertà culturale e di benessere condivise da tutte le componenti della società civile.

    E’ dotata di una forte spiritualità che la porta a studiare e frequentare persone & convegni/seminari che hanno come oggetto di studio la profonda conoscenza delle diverse religioni nell’individualità delle differenze e nei comuni principi etici.

    Vive in campagna, vicino ad Ostuni con marito e 2 cani corso

    More that meets the eye